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The EUROIMMUN Microplate ELISA
Principle of the Test
- Polystyrene microplate strips coated with purified, order biochemically characterized antigens are used as solid phase containing bound antigens.If the sample is positive, specific antibodies in the diluted serum sample attach to the antigens coupled to the solid phase.
- In a second step, the attached antibodies are detected with peroxidase-labeled anti-human antibodies.
- In a third step, the bound antibodies are made visible using a chromogen/substrate solution which promotes a color reaction. The intensity of the color produced is proportional to the concentration of antibodies in the serum sample.
- Monospecific ELISA (enzyme immunoassays with a single antigen) provide a quantitative in-vitro assay for the detection of antibodies. “Profile ELISA” provide a semiquantitative in-vitro assay for the detection of different antibodies on a single microplate strip.
- The solid phase of “Pool ELISA” is coated with an antigen mixture for the semi-quantitative detection of antibodies whose specificity must be investigated subsequently by monospecific assays.