Vasculitis Products

International Consensus Aligned
Fully compliant with 2017 AAV consensus and KDIGO 2024 guidelines for ANCA testing^1,2

Proprietary EUROPLUS™ BIOCHIP
Combines granulocytes (EOH/HCHO) with PR3, MPO, and GBM antigens on a single reaction field

Single Incubation Testing
Parallel observation of patterns on ethanol- and formalin-fixed granulocyte, plus the exclusion of ANA interference on Hep-2³

Innovative PR3-hn-hr ELISA
Unique mixture of human native and human recombinant PR3 for enhanced sensitivity⁴

Background

Autoantibodies to neutrophil cytoplasmic antigens (ANCA) play a central role in the evaluation of small-vessel vasculitides, including granulomatosis with polyangiitis (GPA), microscopic polyangiitis (MPA), and eosinophilic granulomatosis with polyangiitis (EGPA). The principal target antigens in ANCA-associated vasculitis (AAV) are proteinase 3 (PR3) and myeloperoxidase (MPO). Another form of vasculitis of the small blood vessels is anti-GBM disease (Goodpasture syndrome), which affects the glomerular and, in some cases, also the pulmonary capillaries. Characteristic autoantibodies are directed against the glomerular basement membrane (GBM). Up to 35 % of anti-GBM positive samples are also ANCA positive.⁵Due to the clinical overlap of AAV and anti-GBM disease, it is recommended to investigate both anti-GBM antibodies and ANCA in patients with corresponding kidney damage.²

Diagnostics

Euroimmun provides a comprehensive diagnostic portfolio combining cell-based immunofluorescence assays (IFA) with high-quality antigen-specific assays (ELISAs), in line with international AAV consensus recommendations.⁵ IFA is performed on granulocytes fixed with ethanol (EOH) and formaldehyde (HCHO), enabling reliable differentiation of cytoplasmic (cANCA), perinuclear (pANCA) and atypical (xANCA) fluorescence patterns.

To further enhance diagnostic confidence, Euroimmun offers EUROPLUS™ BIOCHIP technology, which combines granulocyte substrates with defined single antigens such as PR3, MPO, and GBM on one reaction field, simplifying interpretation and supporting rapid correlation between patterns and antigen specificity.

For specific antibody identification, Euroimmun offers high-quality test systems such as the innovative Anti-PR3-hn-hr ELISA, consisting of a mixture of human native (hn) and human recombinant (hr) PR3, as well as the Anti-MPO ELISA with highly purified human MPO.

References

Hellmich B, Sanchez-Alamo B, Schirmer JH, et al EULAR recommendations for the management of ANCA-associated vasculitis: 2022 update. Annals of the Rheumatic Diseases 2024;83:30-47.
Kidney Disease: Improving Global Outcomes (KDIGO) ANCA Vasculitis Work Group. KDIGO 2024 Clinical Practice Guideline for the Management of Antineutrophil Cytoplasmic Antibody (ANCA)–Associated Vasculitis. Kidney Int. 2024;105(3 Suppl):S71-S116. doi:10.1016/j.kint.2023.10.008.
https://www.sciencedirect.com/science/article/abs/pii/S0022175909002014
https://pubmed.ncbi.nlm.nih.gov/23800447/
Bossuyt, X., Cohen Tervaert, JW., Arimura, Y. et al. Revised 2017 international consensus on testing of ANCAs in granulomatosis with polyangiitis and microscopic polyangiitis. Nat Rev Rheumatol 13, 683–692 (2017). https://doi.org/10.1038/nrrheum.2017.140

For in vitro diagnostic use.

Method :

Parameter :

Substrate :

Species/Antigen :

Order No. :

Method	Parameter	Substrate	Species/Antigen	Order No.
IFA	Granulocytes (EOH) 10 x 05 (test system)	granulocytes, ethanol-fixed	human	FA 1200-1005
IFA	Granulocytes (EOH) 10 x 10 (test system)	granulocytes, ethanol-fixed	human	FA 1200-1010
IFA	Granulocytes (EOH) 180 x 10 (test system)	granulocytes, ethanol-fixed	human	FA 1200-18010
IFA	Granulocytes (EOH) 20 x 05 (test systems)	granulocytes, ethanol-fixed	human	FA 1200-2005
IFA	Granulocytes (EOH) 20 x 10 (test systems)	granulocytes, ethanol-fixed	human	FA 1200-2010
IFA	Granulocytes (HCHO) 10 x 05 (test system)	granulocytes, formalin-fixed	human	FA 1201-1005
IFA	Granulocytes (HCHO) 10 x 10 (test system)	granulocytes, formalin-fixed	human	FA 1201-1010
IFA	Granulocyte Mosaic 2 cANCA, pANCA, GS-ANA cANCA, pANCA 10 X 05 (test system)	2 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-1005-2
IFA	Granulocyte Mosaic 2 cANCA, pANCA, GS-ANA cANCA, pANCA 10 X 10 (test system)	2 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-1010-2
IFA	Granulocyte Mosaic 2 cANCA, pANCA, GS-ANA cANCA, pANCA 20 X 05 (test system)	2 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-2005-2
IFA	Granulocyte Mosaic 2 cANCA, pANCA, GS-ANA cANCA, pANCA 20 X 05 (test system)	2 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-2010-2
IFA	EUROPLUS Granulocyte Mosaic 22 cANCA, pANCA, GS-ANA cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 10 X 05 (test system)	4 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-1005-22
IFA	EUROPLUS Granulocyte Mosaic 22 cANCA, pANCA, GS-ANA cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 10 X 10 (test system)	4 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-1010-22
IFA	EUROPLUS Granulocyte Mosaic 22 cANCA, pANCA, GS-ANA cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 20 X 05 (test system)	4 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-2005-22
IFA	EUROPLUS Granulocyte Mosaic 25 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cell nuclei (ANA), cANCA, pANCA cANCA, pANCA, EUROPattern glom. basement membrane (GBM), EUROPattern pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 10 x 05 (test system)	6 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO) GBM BIOCHIPs MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-1005-25
IFA	EUROPLUS Granulocyte Mosaic 25 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cell nuclei (ANA), cANCA, pANCA cANCA, pANCA, EUROPattern glom. basement membrane (GBM), EUROPattern pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 10 x 10 (test system)	6 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO) GBM BIOCHIPs MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-1010-25
IFA	EUROPLUS Granulocyte Mosaic 25 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cell nuclei (ANA), cANCA, pANCA cANCA, pANCA, EUROPattern glom. basement membrane (GBM), EUROPattern pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 20 x 05 (test system)	6 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO) GBM BIOCHIPs MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-2005-25
IFA	EUROPLUS Granulocyte Mosaic 25 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cell nuclei (ANA), cANCA, pANCA cANCA, pANCA, EUROPattern glom. basement membrane (GBM), EUROPattern pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 20 x 10 (test system)	6 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO) GBM BIOCHIPs MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-2010-25
IFA	EUROPLUS Granulocyte Mosaic 32 cANCA, pANCA, GS-ANA cANCA, pANCA cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 10 X 05 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-1005-32
IFA	EUROPLUS Granulocyte Mosaic 32 cANCA, pANCA, GS-ANA cANCA, pANCA cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 10 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-1010-32
IFA	EUROPLUS Granulocyte Mosaic 32 cANCA, pANCA, GS-ANA cANCA, pANCA cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 20 X 05 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-2005-32
IFA	EUROPLUS Granulocyte Mosaic 32 cANCA, pANCA, GS-ANA cANCA, pANCA cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 20 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-2010-32
IFA	EUROPLUS Granulocyte Mosaic 32 cANCA, pANCA, GS-ANA cANCA, pANCA cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO) cANCA: proteinase 3 (PR3) 120 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FA 1201-12010-32
IFA	EUROPLUS Granulocyte Mosaic 32 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cANCA, pANCA, EUROPattern cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-1005-32
IFA	EUROPLUS Granulocyte Mosaic 32 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cANCA, pANCA, EUROPattern cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 10 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-1010-32
IFA	EUROPLUS Granulocyte Mosaic 32 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cANCA, pANCA, EUROPattern cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 120 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-12010-32
IFA	EUROPLUS Granulocyte Mosaic 32 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cANCA, pANCA, EUROPattern cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 20 X 05 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-2005-32
IFA	EUROPLUS Granulocyte Mosaic 32 EUROPattern cANCA, pANCA, GS-ANA, EUROPattern cANCA, pANCA, EUROPattern cell nuclei (ANA), cANCA, pANCA pANCA: myeloperoxidase (MPO), EUROPattern cANCA: proteinase 3 (PR3), EUROPattern 20 X 10 (test system)	5 BIOCHIPs per field: granulocytes (EOH) granulocytes (HCHO) HEp-2+granulocytes (EOH) MPO BIOCHIPs PR3 BIOCHIPs	human	FC 1201-2010-32
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 10 X 05 (test sytem)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-1005-13
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 10 X 10 (test system)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-1010-13
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 10 X 50 (test sytem)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-1050-13
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 20 X 05 (test system)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-2005-13
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 20 X 10 (test system)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FA 1201-2010-13
IFA	Granulocyte Mosaic 13 cANCA, pANCA, GS-ANA cell nuclei (ANA), cANCA, pANCA cANCA, pANCA 20 X 10 (single slides)	3 BIOCHIPs per field: granulocytes (EOH) HEp-2+granulocytes (EOH) granulocytes (HCHO)	human	FB 1201-2010-13
ELISA	Anti-PR3-hn-hr ELISA (IgG)	antigen-coated microplate wells	hn: native, human neutrophil granulocytes hr: recombinant, human c-DNA expressed in human cell line	EA 1201-9601-2 G
ELISA	Anti-MPO ELISA (IgG)	antigen-coated microplate wells	native, human neutrophil granulocytes	EA 1211-9601 G
Method	Parameter	Substrate	Species/Antigen	Order No.

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Vasculitis

Background

Diagnostics

Vasculitis products

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Vasculitis

Background

Diagnostics

Vasculitis products

For a list of all the products Euroimmun offers please visit our Product Finder

Contact our Autoimmune product team for further details and information

Contact Us

Follow Us

For a list of all the products
Euroimmun offers please visit our Product Finder

Contact our Autoimmune product team
for further details and information