ChLIA

Advantages of EUROIMMUN ChLIA

Capability to have fully automated processing from primary sample to result using the IDS-iSYS Multi-Discipline Automated System or the IDS-i10
Highest speed – results in just 30 minutes
Ready-for-use reagent cartridges and calibrators with long stability and cooling within the instrument
Automated transmission of quality control data by RFID chip for error-free and quick loading with little manual effort
Quantification based on calibration curves which are stable over several weeks
Broad dynamic analytical range due to high signal intensity

Test principle

Chemiluminescence immunoassays (ChLIA) from EUROIMMUN use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals.

Antibody detection by ChLIA

The antigen-coated magnetic particles are incubated with diluted samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the sample within the measurement range and is automatically converted into a concentration by the instrument.

Antigen detection by ChLIA

The antibody-coated magnetic particles are incubated with the sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the sample within the indicated measurement range and is converted automatically into a concentration by the instrument.