Techniques - Microarray

Microarray

EUROArrays

Advantages of the EUROArray System

  • Multiplex analyses for molecular genetic diagnostics and molecular infection diagnostics
  • BIOCHIP and TITERPLANE technologies for excellent standardization and reliable hybridization of the EUROArrays
  • Ready-for-use PCR reagents for simple, fast, efficient, and error-free workflows
  • Numerous integrated control reagents for high reliability of results, e.g. control of sample quality, sufficient stringency in the hybridization reaction, and absence of cross-contamination
  • Fully automated, standardized evaluation, generation of results, and archiving using the EUROArrayScan software (LIMS connection enabled)
  • No need for DNA isolation with EUROArray Direct kits– the analysis is performed directly on untreated blood
  • Complete process validated according to IVD Directive and CE-marked, from sample preparation to issuing of results

Test principle of the EUROArray System

The EUROIMMUN EUROArray system combines the advantages of the polymerase chain reaction (PCR) and microarray technologies for the detection of genetic markers and direct detection of pathogens. The EUROArrays are based on BIOCHIP technology with specific DNA probes.

Each BIOCHIP consists of 72 DNA spots and allows duplicate determinations of up to 36 different DNA sequences, including controls. Also, each EUROArray slide contains five test fields, enabling the analysis of up to five unique samples in parallel. Several BIOCHIPs may undergo a coating on one field to increase the number of detectable DNA sequences.

Technology

For some parameters, EUROIMMUN offers a direct procedure eliminating the need for DNA isolation from the sample. The blood undergoes incubation with the extraction solutions included in the kit. Afterward, you can use it directly for PCR.

With all other parameters, a DNA isolation kit allows for the conventional extraction of DNA. All PCR reagents supplied in EUROArray kits are ready to use, including the DNA polymerase and the validated specific primers.

Using PCR, the specific genetic sequences of the patient DNA are amplified and the PCR products labeled with a fluorescent dye during their formation. In the hybridization (incubation) of the PCR products with the EUROArray, these bind to the complementary probes of the EUROArray. Moreover, the DNA microarray hybridization happens under exact, standardized conditions using the proven TITERPLANE technology. After the reading of the EUROArray in the EUROArrayScanner, the evaluation of fluorescent signals happens automatically using the EUROArrayScan software.

For product information, please contact the EUROIMMUN Sales Team

Automation

EUROArrayScan System

Fully automated evaluation for standardized, fast and reliable results of EUROIMMUN microarrays

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