Microarray

The EUROIMMUN EUROArray system combines the advantages of the polymerase chain reaction (PCR) and microarray technologies for the detection of genetic markers and direct detection of pathogens. The EUROArrays are based on BIOCHIP technology with specific DNA probes.

Each BIOCHIP consists of 72 DNA spots and allows duplicate determinations of up to 36 different DNA sequences, including controls. Also, each EUROArray slide contains five test fields, enabling the analysis of up to five unique samples in parallel. Several BIOCHIPs may undergo a coating on one field to increase the number of detectable DNA sequences.

Technology

For some parameters, EUROIMMUN offers a direct procedure eliminating the need for DNA isolation from the sample. The blood undergoes incubation with the extraction solutions included in the kit. Afterward, you can use it directly for PCR.

With all other parameters, a DNA isolation kit allows for the conventional extraction of DNA. All PCR reagents supplied in EUROArray kits are ready to use, including the DNA polymerase and the validated specific primers.

Using PCR, the specific genetic sequences of the patient DNA are amplified and the PCR products labeled with a fluorescent dye during their formation. In the hybridization (incubation) of the PCR products with the EUROArray, these bind to the complementary probes of the EUROArray. Moreover, the DNA microarray hybridization happens under exact, standardized conditions using the proven TITERPLANE technology. After the reading of the EUROArray in the EUROArrayScanner, the evaluation of fluorescent signals happens automatically using the EUROArrayScan software.