Connective tissue Products - Rheumatology

Gold standard ANA detection
IFA on HEp-2/HEp-2010 substrate is considered the “gold standard” for primary ANA detection ¹

Proprietary BIOCHIP technology
Allows for ANA detection on either HEp-2 or HEp-20-10 cell substrates, in a single miniature field

Unique HEp-20-10 Substrate
An increased number of mitotic cells enhances pattern interpretation and differentiation ³

Multiple testing platforms
ELISA, IFA, and EUROLINE immunoblots for flexible workflow integration

Background

Connective tissue diseases (CTDs) are rheumatic diseases characterized by damage to the body’s connective tissues, including joints, muscles, skin, and internal organs. Autoantibodies against cellular components, including anti-nuclear antibodies (ANA), are important serological markers for CTD. Euroimmun delivers solutions to support ANA detection for efficient diagnosis of several CTD including:

Systemic lupus erythematosus (SLE)
Systemic sclerosis (SSc)
Sjögren’s syndrome (SS)
Idiopathic inflammatory myopathies (Myositis)

Diagnostics

Indirect immunofluorescence assay (IFA) on HEp-2 or HEp-20-10 cells remains the gold standard for ANA detection due to its high sensitivity and ability to reveal characteristic fluorescence patterns. The international consensus on ANA patterns (ICAP, www.anapatterns.org) defines nuclear, cytoplasmic, and mitotic patterns that guide clinical interpretation. ^1,2

Euroimmun’s BIOCHIP technology allows for ANA detection on either HEp-2 or HEp-20-10 cells substrates, in a single miniature field. Unique to Euroimmun, the HEp-20-10 substrate provides a higher number of mitotic cells, aiding pattern interpretation and differentiation.³

Additionally, Euroimmun provides a range of solid-phase assays, including ELISA and EUROLINE immunoblots, covering autoantibodies relevant to SLE, SSc, SS, and myositis. These assays detect antibodies such as anti-dsDNA, anti-Sm, anti-SSA/SSB, anti-Scl-70, anti-centromere, anti-Jo-1, ribosomal P, and Ro-52.

References

Edward K L Chan, Carlos A von Mühlen, Marvin J Fritzler, Jan Damoiseaux, Maria Infantino, Werner Klotz, Minoru Satoh, Lucile Musset, Ignacio García-De La Torre, Orlando Gabriel Carballo, Manfred Herold, Wilson de Melo Cruvinel, Tsuneyo Mimori, Karsten Conrad, Luis E C Andrade, the ICAP Committee, The International Consensus on ANA Patterns (ICAP) in 2021—The 6^thWorkshop and Current Perspectives, The Journal of Applied Laboratory Medicine, Volume 7, Issue 1, January 2022, Pages 322–330, https://doi.org/10.1093/jalm/jfab140.
Damoiseaux, J., von Mühlen, C.A., Garcia-De La Torre, I. et al.International consensus on ANA patterns (ICAP): the bumpy road towards a consensus on reporting ANA results. Autoimmun Highlights7, 1 (2016). https://doi.org/10.1007/s13317-016-0075-0.
Rohwäder, E., Locke, M., Fraune, J., & Fechner, K. (2015). Diagnostic profile on the IFA 40: HEp-20-10 – an immunofluorescence test for reliable antinuclear antibody screening. Expert review of molecular diagnostics, 15(4), 451–462. https://doi.org/10.1586/14737159.2015.993612.

Connective tissue diseases products

For in vitro diagnostic use.

Method :

Parameter :

Substrate :

Species/Antigen :

Order No. :

Method	Parameter	Substrate	Species/Antigen	Order No.
ELISA	Anti-ENA pool ELISA (IgG)	Antigen-coated microplate wells	7 antigens; antigen mixture: nRNP/Sm, Sm, SS-A, SS-B, Scl-70, ribosomal P-proteins, Ro-52	EA 1590-9601-9 G
ELISA	ANA Screen ELISA (IgG)	Antigen-coated microplate wells	10 ANA antigens antigen mixture: dsDNA, histones, ribosomal P-proteins, nRNP/Sm, Sm, SS-A, SS-B, Scl-70, Jo-1, centromeres	EA 1590-9601-8 G
ELISA	Anti-nRNP/Sm ELISA (IgG)	Antigen-coated microplate wells	nRNP/Sm	EA 1591-9601 G
ELISA	Anti-dsDNA-NcX ELISA (IgG)	Antigen-coated microplate wells	dsDNA-NcX	EA 1572-9601 G
ELISA	Anti-SS-B ELISA (IgG)	Antigen-coated microplate wells	SS-B (La)	EA 1597-9601 G
ELISA	Anti-Scl-70 ELISA (IgG)	Antigen-coated microplate wells	Scl-70	EA 1599-9601-G
ELISA	Anti-Centromeres ELISA (IgG)	Antigen-coated microplate wells	centromeres	EA 1611-9601 G
ELISA	Anti-ribosomal P proteins ELISA (IgG)	Antigen-coated microplate wells	ribosomal P-proteins	EA 1641-9601 G
ELISA	Anti-Jo-1 ELISA (IgG)	Antigen-coated microplate wells	Jo-1	EA 1661-9601 G
ELISA	Anti-dsDNA-NcX ELISA	Antigen-coated microplate wells	dsDNA and nucleosomes	EA-1572-9601 G
IFA	IFA 40: HEp-20-10	HEp-20-10 Cells	human	FA 1522-XXXX-1
IFA	ANA IFA: HEp-20-10	HEp-20-10 Cells	human	FA 1522-XXXX
IFA	IFA 40: HEp-20-10 EUROPattern	HEp-20-10 Cells	human	FC 1522-XXXX-1
IFA	CLIFT (anti-dsDNA) EUROPattern	Flagellates	Crithidia luciliae	FC 1572- XXXX
IFA	CLIFT sensitive (anti-dsDNA)	Flagellates	Crithidia luciliae	FA 1572- XXXX-1
IFA	CLIFT sensitive (anti-dsDNA) EUROPattern	Flagellates	Crithidia luciliae	FC 1572- XXXX-1
EUROLINE	EUROLINE ENA ProfilePlus 4	EUROLINE	nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70,ribosomal P-proteins	DL 1590-4 G
EUROLINE	EUROLINE ENA Profile 9 Ag	EUROLINE	nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70,Jo-1, CENP B, ribosomal P-proteins	DL 1590-9 G
Method	Parameter	Substrate	Species/Antigen	Order No.

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Connective tissue diseases

Background

Diagnostics

Connective tissue diseases products

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Euroimmun offers please visit our Product Finder

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Connective tissue diseases

Background

Diagnostics

Connective tissue diseases products

For a list of all the products Euroimmun offers please visit our Product Finder

Contact our Autoimmune product team for further details and information

Contact Us

Follow Us

For a list of all the products
Euroimmun offers please visit our Product Finder

Contact our Autoimmune product team
for further details and information