Products - Autoimmune - Hepatology - Autoimmune hepatitis

Autoimmune hepatitis

Contact our Autoimmune Product Team for further details and information

Disease information

Autoimmune hepatitis or AIH (also called as lupoid hepatitis) is a disease that predominantly affects females (75% of cases). It involves destruction of hepatic parenchyma (liver cells called hepatocytes) by various autoantibodies produced by the body. The histology of autoimmune hepatitis reflects widespread necrosis and an increased lymphocytic infiltrate. Lab assays show elevated serum bilirubin, hepatic enzymes like ALT and rising antibody titers.

Although, autoimmune hepatitis (AIH) can affect any age group, the incidence peak is usually seen in young to middle aged segments of the population. In Western Europe, AIH affects 1.9 per 100,000 people per year which is fairly high when compared to world standards. Hence, adequate screening and diagnostic workup are the only keys to slow down the rising curve.

Diagnostic Guidelines

All autoimmune diseases can be detected on the basis of high levels of circulating antibodies against self-antigens, this is true for autoimmune hepatitis (AIH) as well. We expect to find high titers of following antibodies in your blood:

  • Antibodies against cell nuclei (ANA), native DNA
  • Smooth muscle (ASMA, most important target antigen: F-actin)
  • Soluble liver antigen/ liver-pancreas antigen (SLA/LP)
  • Liver-kidney microsomes (LKM-1, target antigen: cytochrome P450 IID6)
  • Liver cytosolic antigen type 1 (LC-1, target antigen: formiminotransferase cyclodeaminase).

The SLA/LP antibody titers that are detected by our EUROIMMUN enzyme immunoassays hold the highest accuracy for diagnosing AIH. These antibodies are seen either alone or in conjunction with other antibodies. Although their prevalence stacks around 10 to 30%, their predictive value is a 100 percent.

Another important predictor of AIH is ASMA antibodies. These are directed against F-actin and since the sampling involves frozen tissues or cells, these cannot be detected by conventional ELISA or Western blot techniques. Our specially designed immunofluorescence assays are highly efficient in detecting a hike in these antibodies and thus facilitate in the accurate diagnosis of AIH.

Autoimmune hepatitis products

The individual product regulatory statements may vary, please refer to the instructions for use for more information.

wdt_ID Method Parameter Substrate Species/ Antigen
347 IFA liver-specific
antigens
liver monkey
348 EUROLINE positive control serum: IgG, human, 100x concentrated
for DL 1300-X G
349 EUROASSAY AMA M2, LKM-1, SLA/LP EUROASSAY strip
with antigens
350 IFA Liver Mosaic 1
liver-kidney microsomes (LKM), ANA
mitochondria (AMA), LKM
2 BIOCHIPs per field:
liver
kidney
rat
rat
351 IFA Liver Mosaic 2
liver antigens, cell nuclei (ANA)
AMA M7, heart antigens
cell nuclei (ANA)
LKM, ANA
mitochondria (AMA), LKM
smooth muscles (ASMA)
6 BIOCHIPs per field:
liver
heart
HEp-2 cells
liver
kidney
stomach
monkey
monkey
human
rat
rat
rat
352 EUROLINE Liver Profile 2
(AMA M2, M2-3E, LKM-1, LC-1, SLA/LP separately)
EUROLINE
353 EUROASSAY LKM-1, LC-1, SLA/LP EUROASSAY strip
with antigens
354 IFA Liver Sreen 1
mitochondria (AMA), LKM
soluble liver antigen/
liver-pancreas antigen (SLA/LP)
LKM, ANA
smooth muscles (ASMA)
4 BIOCHIPs per field:
kidney
transfected cells
liver
stomach
rat
EU 90
rat
rat
355 IFA Liver Mosaic 3
liver antigens, cell nuclei (ANA)
skeletal muscle
cell nuclei (ANA)
LKM, cell nuclei (ANA)
mitochondria (AMA), LKM
smooth muscles (ASMA)
6 BIOCHIPs per field:
liver
musculus iliopsoas
HEp-2 cells
liver
kidney
stomach
monkey
monkey
human
rat
rat
rat
356 EUROLINE Liver Profile
(AMA M2, LKM-1, LC-1, SLA/LP separately)
EUROLINE
357 EUROASSAY Liver Profile
AMA M2, LKM-1, LC-1, SLA/LP
EUROASSAY strip
with antigens
358 EUROLINE Autoimmune Liver Diseases
(AMA M2, M2-3E, Sp100, PML, gp210,
LKM-1, LC-1, SLA/LP, Ro-52 separately)
EUROLINE
359 EUROLINE Autoimmune Liver Diseases 14 Ag
(AMA-M2, M2-3E, Sp100, PML, gp210,
LKM-1, LC-1, SLA/LP, SS-A, Ro-52, Scl-70,
CENP A, CENP B, PGDH separately)
EUROLINE
360 IFA Autoimmune liver diseases Screen 8
EUROPattern
liver antigens, cell nuclei (ANA)
F-actin
cell nuclei (ANA), EUROPattern
LKM, ANA
mitochondria (AMA), LKM
smooth muscles (ASMA)
6 BIOCHIPs per field:
liver
VSM47
HEp-2 cells
liver
kidney
stomach
monkey
rat
human
rat
rat
rat
361 IFA Liver Mosaic 8
liver antigens, cell nuclei (ANA)
F-actin
cell nuclei (ANA)
LKM, ANA
mitochondria (AMA), LKM
smooth muscles (ASMA)
6 BIOCHIPs per field:
liver
VSM47
HEp-2 cells
liver
kidney
stomach
monkey
rat
human
rat
rat
rat
362 IFA Autoimmune liver diseases Screen 9
EUROPattern
mitochondria (AMA), LKM
LKM, ANA
smooth muscles (ASMA)
F-actin
4 BIOCHIPs per field:
kidney
liver
stomach
VSM47
rat
rat
rat
rat
363 IFA Liver Mosaic 9
mitochondria (AMA), LKM
LKM, ANA
smooth muscles (ASMA)
F-actin
4 BIOCHIPs per field:
kidney
liver
stomach
VSM47
rat
rat
rat
rat
364 IFA antibodies against soluble liver antigen/
liver-pancreas antigen (SLA/LP ab control)
365 ELISA soluble liver antigen/
liver-pancreas antigen (SLA/LP)
antigen-coated
microplate wells
recombinant SLA/LP
expression in E. coli
366 EUROASSAY SLA/LP EUROASSAY strip
with antigens
367 IFA soluble liver antigen/
liver-pancreas antigen (SLA/LP)
transfected cells
control transfection
(2 BIOCHIPs per field)
EU 90
EU 90
368 ELISA cytosolic
liver antigen type 1
(LC-1)
antigen-coated
microplate wells
recombinant formiminotransferase
cyclodeaminase, expression with
baculovirus vector in insect cells
369 IFA antibodies against liver-kidney microsomes
(LKM ab control)
370 ELISA liver-kidney microsomes
(LKM-1)
antigen-coated
microplate wells
recombinant cytochrome
P450 IID6
371 IFA antibodies against F-actin
Method Parameter Substrate Species/ Antigen
For product information, please contact the EUROIMMUN Sales Team

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