Products - Autoimmune - Rheumatology - Anti-phospholipid syndrome

Anti-phospholipid syndrome

Contact our Autoimmune Product Team for further details and information

Disease information

Anti-phospholipid syndrome which also goes by the name, Hughes syndrome, is an immune disorder characterized by an increased risk for blood clots. The autoantibodies formed cause blood to clot in the arteries of several different organ systems of the body resulting in thrombosis and organ ischemia.

The first official criteria for anti-phospholipid syndrome was drafted in 1998 where it was agreed the disease will be considered if at least one clinical and one serological criteria are met. The clinical criteria includes vascular thrombosis and pregnancy complications such as premature births, spontaneous abortions and eclampsia. The criteria got updated in 2004. Now the disease diagnosis is based on the following findings:

  • Antibodies against cardiolipin (ACA; IgG or IgM)
  • Antibodies against beta 2 glycoprotein 1
  • Or a positive lupus anticoagulant (LA) test.

In serological diagnosis, antibodies of several different classes show up. Moreover, a key observation is that around 10% of the healthy individuals too have anti-phospholipid antibodies as these can be induced by infections etc. too. Hence, an adequate diagnosis stems from a parallel clinical and serological testing.

Diagnostic Guidelines

For detection of APLAs, there’s no better test than ELISA.

We offer microtiter (highly sensitive) ELISAs for detection of antibodies against cardiolipin, beta 2 glycoprotein 1 and phosphatidylserine. Plus EUROIMMUN also enables the possibility of detecting the immunoglobulin classes IgG, IgA and IgM separately as well as simultaneously (IgAGM). Moreover, lupus anticoagulant test can also be done using a multistage process in compliance with the guidelines of International Society of Thrombosis and Hemostasis. The test used although highly specific ranks a little low on sensitivity. Hence variable results are expected.

EUROIMMUN microtiter ELISAs for detection of antibodies against cardiolipin and beta 2 glycoprotein 1 have shown very high sensitivity in clinical studies. Sera from patients with viral hepatitis or parvovirus B19 infections and sera from healthy blood donors demonstrated only 0 to 2% positive results, while in studies using tests from other manufacturers values of between 12 and 50% were obtained. Antibodies against cardiolipin are highly indicative of anti-phospholipid syndrome. In a cohort of 1000 patients, 88% were positive for these antibodies. Our special ELISAs can easily detect even the slightest titers of these antibodies to help draft and accurate diagnosis.

Anti-phospholipid syndrome products

The individual product regulatory statements may vary, please refer to the instructions for use for more information.

wdt_ID Method Parameter Substrate Species/ Antigen
340 ELISA cardiolipin (AMA M1) antigen-coated
microplate wells
native,
bovine heart
341 ELISA cardiolipin (AMA M1) antigen-coated
microplate wells
native,
bovine heart
342 ELISA cardiolipin (AMA M1) antigen-coated
microplate wells
native,
bovine heart
343 ELISA cardiolipin (AMA M1) antigen-coated
microplate wells
native,
bovine heart
344 ELISA phosphatidylserine antigen-coated
microplate wells
native,
bovine brain
345 ELISA ß2-glycoprotein 1 antigen-coated
microplate wells
native,
human serum
346
Method Parameter Substrate Species/ Antigen
For product information, please contact the EUROIMMUN Sales Team

Product Catalog

Download our full EUROIMMUN US 2022 Product Catalog (PDF 12MB)

Download PDF

2022 EUROIMMUN US

Privacy Policy | Sitemap