Products - Autoimmune - Rheumatology - Vasculitis

Vasculitis

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Disease information

Many of the medium and small vessel vasculitis share an autoimmune etiology. Consider some examples:

  • Wegener’s granulomatosis
  • Microscopic polyangiitis (MPA)
  • Eosinophilic granulomatosis with polyangiitis (EGPA)

All these are associated with ANCA (antineutrophil cytoplasmic antibodies). These antibodies attack antigens found in the cytoplasmic granules of neutrophils and monocytes and serve as an important serological marker for autoimmune (ANCA-associated) vasculitis.

Patients with glomerulonephritis in which antibodies are formed against the glomerular basement membranes, also turn out positive for serum ANCAs. Hence its important to double check the patients positive for anti-GBM antibodies, to look for any associated antineutrophil cytoplasmic antibodies indicative of autoimmune vasculitis.

Diagnostic Guidelines

Diagnostics for ANCA associated vasculitis (AAV) are primarily based upon immunofluorescence (IFA). Standard for IFA is a BIOCHIP of ethanol and formaldehyde fixed human granulocytes. However, special EUROIMMUN BIOCHIPs (e.g. HEp2 cells) also play a significant role in increasing the diagnostic certainty. Our technique integrates conventional cell culture substrates with defined single antigens on one test field. This makes it easy to interpret different immunofluorescence patterns and thus increases the diagnostic value.

Types of ANCA

On immunofluorescence, two types of ANCA are recognized:

  • Cytoplasmic type (cANCA) which is directed against proteinase 3 (PR3)
  • And perineural type (pANCA) which is indicative of a spectrum of diseases.

The main target antigens for pANCA are myeloperoxidases (found in the cytoplasmic granules of myelocytes). However, granulocyte elastase, lactoferrin, cathapsin and lyzozyme etc. also act as potential target antigens.

All diagnostics for anti-PR3 and anti-MPOs should be confirmed with monospecific test such as ELISA/ChLIA. Moreover, most of the times, the antibodies are not detected on ELISA. Hence, sensitivity and specificity of the tests shoulder over parallel performance of both IFA and ELISA.

Vasculitis products

The individual product regulatory statements may vary, please refer to the instructions for use for more information.

wdt_ID Method Parameter Substrate Species/ Antigen
144 IFA antibodies against granulocyte cytoplasm,
cytoplasmic pattern (cANCA control, PR3 ab control)
145 IFA cytoplasm of granulocytes (cANCA,
pANCA), nuclei of granulocytes (GS-ANA)
granulocytes,
ethanol-fixed
human
146 IFA cytoplasm of granulocytes (cANCA,
pANCA), nuclei of granulocytes (GS-ANA)
EUROPattern
granulocytes,
ethanol-fixed
human
147 IFA antibodies against granulocyte cytoplasm,
aliquots from positive control for ANCA diagnostics
(10 different fluorescence patterns)
148 ELISA ANCA Profile (proteinase 3, MPO, elastase,
cathepsin G, BPI, lactoferrin separately)
antigen-coated
microplate wells
native, human
neutrophil granulocytes
recombinant, human c-DNA
expressed in human cell line
149 EUROLINE myeloperoxidase (MPO)
proteinase 3 (PR3)
EUROLINE
150 EUROLINE positive control serum: IgG, human, 100x concentrated
for DL 1200-X G
151 IFA antibodies against granulocyte cytoplasm, cytoplasmic pattern
(cANCA control, PR3 ab control), control serum with indication of titer
152 EUROLINE myeloperoxidase (MPO)
proteinase 3 (PR3)
glomerular basement membrane (GBM)
EUROLINE
153 IFA granulocytes
(cANCA, pANCA)
granulocytes,
formaldehyde-fixed
human
154 IFA granulocytes
(cANCA, pANCA)
EUROPattern
granulocytes,
formaldehyde-fixed
human
155 ChLIA control set
Proteinase 3 (PR3)
156 ChLIA proteinase 3 (PR3)
157 IFA Granulocyte Mosaic 13 EUROPattern
cANCA, pANCA, GS-ANA, EUROPattern
cell nuclei (ANA), cANCA, pANCA
cANCA, pANCA, EUROPattern
3 BIOCHIPs per field:
granulocytes (EOH)
HEp-2+granulocytes (EOH)
granulocytes (HCHO)
human
human
human
158 IFA Granulocyte Mosaic 13
cANCA, pANCA, GS-ANA
cell nuclei (ANA), cANCA, pANCA
cANCA, pANCA
3 BIOCHIPs per field:
granulocytes (EOH)
HEp-2+granulocytes (EOH)
granulocytes (HCHO)
human
human
human
159 IFA Granulocyte Mosaic 15 EUROPattern
cANCA, pANCA, GS-ANA, EUROPattern
cell nuclei (ANA), cANCA, pANCA
2 BIOCHIPs per field:
granulocytes (EOH)
HEp-2+granulocytes (EOH)
human
human
160 IFA Granulocyte Mosaic 15
cANCA, pANCA, GS-ANA
cell nuclei (ANA), cANCA, pANCA
2 BIOCHIPs per field:
granulocytes (EOH)
HEp-2+granulocytes (EOH)
human
human
161 IFA Granulocyte Mosaic 17
cANCA, pANCA, GS-ANA
cANCA, pANCA
cANCA, pANCA
cell nuclei (ANA), cANCA, pANCA
4 BIOCHIPs per field:
granulocytes (EOH)
granulocytes (HCHO)
granulocytes (MOH)
HEp-2+granulocytes (EOH)
human
human
human
human
162 IFA Granulocyte Mosaic 2 EUROPattern
cANCA, pANCA, GS-ANA, EUROPattern
cANCA, pANCA, EUROPattern
2 BIOCHIPs per field:
granulocytes (EOH)
granulocytes (HCHO)
human
human
163 IFA Granulocyte Mosaic 2
cANCA, pANCA, GS-ANA
cANCA, pANCA
2 BIOCHIPs per field:
granulocytes (EOH)
granulocytes (HCHO)
human
human
164 ELISA cANCA:
proteinase 3
(PR3-hn-hr)
antigen-coated
microplate wells
hn: native, human
neutrophil granulocytes
hr: recombinant, human c-DNA
expressed in human cell line
165 IFA EUROPLUS Granulocyte Mosaic 20
cANCA, pANCA, GS-ANA
pANCA: myeloperoxidase (MPO)
cANCA: proteinase 3 (PR3)
3 BIOCHIPs per field:
granulocytes (EOH)
MPO BIOCHIPs
PR3 BIOCHIPs
human
166 IFA EUROPLUS Granulocyte Mosaic 22
cANCA, pANCA, GS-ANA
cANCA, pANCA
pANCA: myeloperoxidase (MPO)
cANCA: proteinase 3 (PR3)
4 BIOCHIPs per field:
granulocytes (EOH)
granulocytes (HCHO)
MPO BIOCHIPs
PR3 BIOCHIPs
human
human
167 IFA EUROPLUS Granulocyte Mosaic 22
EUROPattern
cANCA, pANCA, GS-ANA, EUROPattern
cANCA, pANCA, EUROPattern
pANCA: myeloperoxidase (MPO), EUROPattern
cANCA: proteinase 3 (PR3), EUROPattern
4 BIOCHIPs per field:
granulocytes (EOH)
granulocytes (HCHO)
MPO BIOCHIPs
PR3 BIOCHIPs
human
human
168 IFA EUROPLUS Granulocyte Mosaic 25
cANCA, pANCA, GS-ANA
cell nuclei (ANA), cANCA, pANCA
cANCA, pANCA
glomerular basement membrane (GBM)
pANCA: myeloperoxidase (MPO)
cANCA: proteinase 3 (PR3)
6 BIOCHIPs per field:
granulocytes (EOH)
HEp-2+granulocytes (EOH)
granulocytes (HCHO)
GBM BIOCHIPs
MPO BIOCHIPs
PR3 BIOCHIPs
human
human
human
Method Parameter Substrate Species/ Antigen
Contact our Autoimmune Product Team for further details and information

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