Products - Autoimmune - Rheumatology - Connective tissue

Connective tissue

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Information

Connective tissue diseases (CTD) are rheumatic diseases. They include, for example, systemic lupus erythematosus, systemic sclerosis, Sj√∂gren’s syndrome, myositis syndromes or Sharp syndrome.
Autoantibodies against cellular components, commonly called anti-nuclear antibodies (ANA), are important serological markers for CTD. Target antigens include nucleic acids, cell nuclear proteins and ribonuclear proteins as well as components of the cytoplasm. The frequency (prevalence) of ANA in inflammatory rheumatic diseases lies between 20% and 100%. Differential ANA diagnostics is therefore indispensable for the identification of individual rheumatic diseases and their differentiation from other autoimmune diseases.

Diagnostics

The gold standard for the determination of ANA is the indirect immunofluorescence test (IIFT) with human epithelial cells (HEp-2), which is known for its high sensitivity and specificity. In the microscopic evaluation it is possible to establish precisely how an indicator dye (generally fluorescein) is distributed in the cells. Depending on the location of the individual autoantigens, a typical fluorescence pattern for every type of autoantibody bound will appear.
The revised international consensus on a standardized nomenclature of HEp-2 cell patterns in indirect immunofluorescence (ICAP, www.anapatterns.org) defines 15 nuclear, 9 cytoplasmic and 5 mitotic patterns which are relevant for the diagnostics of various autoimmune diseases.

Furthermore, ICAP recommends IIFT results be confirmed by additional specific antibody tests (e.g. ELISA, line blot, ChLIA). However, the exclusive use of these monospecific test methods is insufficient for the determination of autoantibodies against cell nuclei, as not all relevant antigens are available yet in purified form. Thus, the corresponding ANAs can only be detected by IIFT.

Connective tissue products

For Research Use Only. Not For Use In Diagnostic Procedures.
The individual product regulatory statements may vary, please refer to the instructions for use for more information.

wdt_ID Method Parameter Substrate Species/ Antigen
186 IFA cell nuclei (ANA) EUROPattern
cell nuclei (ANA)
cell nuclei (ANA)
HEp-2 cells
liver
(2 BIOCHIPs per field)
human
monkey
187 IFA cell nuclei
(ANA global test)
HEp-2 cells
liver
(2 BIOCHIPs per field)
human
monkey
188 IFA EUROPLUS ANA Mosaic 10
cell nuclei (ANA)
SS-A + SS-B
2 BIOCHIPs per field:
HEp-2 cells
SS-A+SS-B BIOCHIPs
human
189 IFA ANA Mosaic 2 EUROPattern
cell nuclei (ANA), EUROPattern
mitochondria (AMA)
2 BIOCHIPs per field:
HEp-2 cells
kidney
human
rat
190 IFA cell nuclei (ANA)
mitochondria (AMA
HEp-2 cells
kidney
(2 BIOCHIPs per field)
human
rat
191 IFA cell nuclei (ANA) EUROPattern
cell nuclei (ANA)
HEp-20-10 cells
liver
(2 BIOCHIPs per field)
human
monkey
192 IFA cell nuclei
(ANA global test)
HEp-20-10 cells
liver
(2 BIOCHIPs per field)
human
monkey
193 IFA EUROPLUS ANA Mosaic 10A
cell nuclei (ANA)
SS-A + SS-B
2 BIOCHIPs per field:
HEp-20-10 cells
SS-A+SS-B BIOCHIPs
human
194 IFA cell nuclei (ANA)
mitochondria (AMA)
HEp-20-10 cells
kidney
(2 BIOCHIPs per field)
human
rat
195 IFA EUROPLUS ANA Mosaic 20A
cell nuclei (ANA)
cell nuclei (ANA)
SS-A + SS-B
ribosomal P-proteins + Jo-1
4 BIOCHIPs per field:
HEp-20-10 cells
liver
SS-A+SS-B BIOCHIPs
rib. P-prot.+Jo-1 BIOCHIPs
human
monkey
196 IFA EUROPLUS ANA Mosaic 22A
cell nuclei (ANA)
cell nuclei (ANA)
nRNP/Sm + Sm + SS-A
SS-B + Scl-70 + Jo-1
4 BIOCHIPs per field:
HEp-20-10 cells
liver
nRNP/Sm+Sm+SS-A BIOCHIPs
SS-B+Scl-70+Jo-1 BIOCHIPs
human
monkey
197 IFA cell nuclei (ANA)
cell nuclei (ANA)
HEp-20-10 cells
liver
(2 BIOCHIPs per field)
human
rat
198 IFA cell nuclei (ANA) HEp-2 cells human
199 IFA cell nuclei (ANA)
EUROPattern
HEp-2 cells human
200 IFA cell nuclei (ANA) HEp-20-10 cells human
201 IFA cell nuclei (ANA)
EUROPattern
HEp-20-10 cells human
202 EUROLINE Myositis Profile
(Mi-2, Ku, PM-Scl, Jo-1, PL-7,
PL-12, Ro-52 separately)
EUROLINE
203 EUROLINE positive control serum: IgG, human, 100x concentrated
for DL 1530-X G
204 EUROLINE Myositis Profile 3
(Mi-2, Ku, PM-Scl100, PM-Scl75, Jo-1, SRP,
PL-7, PL-12, EJ, OJ, Ro-52 separately)
EUROLINE
205 EUROLINE Autoimmune Inflammatory Myopathies 16 Ag
(Mi-2 alpha, Mi-2 beta, TIF1g, MDA5, NXP2, SAE1, Ku, PM-Scl100,
PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ, Ro-52 separately)
EUROLINE
206 EUROLINE Autoimmune Inflammatory Myopathies 16 Ag et cN-1A
(Mi-2 alpha, Mi-2 beta, TIF1g, MDA5, NXP2, SAE1, Ku,
PM-Scl100, PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ,
Ro-52, cN-1A separately)
EUROLINE
207 EUROLINE positive control serum: IgG, human, 100x concentrated
for DL 1532 G
208 EUROLINE Systemic Sclerosis Profile (Nucleoli)
(Scl-70, CENP A, CENP B, RP11, RP155,
fibrillarin, NOR90, Th/To, PM-Scl100, PM-Scl75,
Ku, PDGFR, Ro-52 separately)
EUROLINE
209 ELISA histones antigen-coated
microplate wells
native, highly purified histones,
bovine thymus
210 IFA antibodies against cell nuclei
(ANA control), homogeneous pattern
Method Parameter Substrate Species/ Antigen
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