Zika Virus

The RNA genome of ZIKV can be directly detected up to 5 to 10 days after onset of symptoms in serum and up to 14 to 21 days in urine by means of polymerase chain reaction (PCR). Specific antibodies against ZIKV, in contrast, are first detected several days after onset of syptoms. Therefore, direct detection of ZIKV RNA plays an important role especially in early stages of the infection, while serological antibody detection is especially relevant for the diagnosis of infections at later stages. Owing to detection based on RNA, ZIKV infections can be clearly distinguished from other viral infections e.g. dengue or chikungunya virus, which cause similar symptoms and are endemic in the same regions.

The EURORealTime Zika Virus test provides highly specific and sensitive direct detection of ZIKV by means of reverse transcriptase real-time PCR. Reverse transcription, amplification, and detection of ZIKV cDNA take place in a single reaction. Raw data analysis, interpretation of data, creation of reports, and electronic archiving of results, taking into account all internal and external controls, are performed fully automatically by the EURORealTime Analysis Software. The data processing steps required in real-time PCR analyses are thus significantly simplified and accelerated. Moreover, the software conveniently guides the user through the entire workflow, helping to prevent mistakes.

EUROIMMUN offers the complete range of test systems for specific detection of ZIKV infections, both for direct detection by real-time PCR and serological detection of specific antibodies.